• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TNFSF15 (R32A, R85A) Trimer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
  • Amino Acid Range: Asp91-Leu251
Catalog #FSF-HM12M
Price
Regular price $39000
Regular price Sale price $39000
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Size
Avantor VWR Fisher Scientific

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Human TNFSF15 (R32A, R85A) Trimer Protein (FSF-HM12M) Summary

Product Description

Recombinant Human TNFSF15 (R32A, R85A) Trimer Protein is expressed from HEK293 with His tag at the N-terminus.It contains Asp91-Leu251.

Product Background

TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Human TNFSF15 (R32A, R85A) Trimer Protein (FSF-HM12M) Specifications/Details

Protein
TNFSF15
Synonyms
TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A;VEGI251
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Asp91-Leu251
Molecular Weight
The protein has a predicted MW of 56.92 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human TNFSF15 (R32A, R85A) Trimer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
Product Tag
N-His
Fluorophore
Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human TNFSF15 (R32A, R85A) Trimer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. Immobilized Human TNFSF15 (R32A, R85A) Trimer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
Human TNFSF15 (R32A, R85A) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TNFSF15 (R32A, R85A) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFSF15 (R32A, R85A) Trimer is greater than 95% as determined by SEC-HPLC. The purity of Human TNFSF15 (R32A, R85A) Trimer is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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