Name: Human tPA Protein (TPA-HM10A)
Brand: KactusBio
SKU: TPA-HM10A-100UG
Price: 270.0 USD
Availability: InStock

Catalog #TPA-HM10A

Size

100UG

500UG

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Product Description	Recombinant Human tPA Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser36-Pro562, which consists of two chains: chain A (Ser36-Arg310) and chain B (Ile311-Pro562).
Product Background	Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.
Product Category	Recombinant Protein / Antibody discovery

Protein	tPA
Synonyms	t-PA; tPA; Alteplase; Reteplase; PLAT;
Accession	P00750-1
Species	Human
Biotinylated	no
Amino Acid Range	Ser36-Pro562
Molecular Weight	The protein has a predicted MW of 60.13 kDa. The protein is activated by thermolysin and cleaved to a 2-chain protease, it migrates to 40-45 kDa (Chain A) and 35-40 kDa (Chain B) based on Bis-Tris PAGE result.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5).
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.