Name: Human Transferrin Protein (TFN-HM101)
Brand: KactusBio
SKU: TFN-HM101-100UG
Price: 165.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 0.50ug/ml determined by ELISA (QC Test). The affinity constant of 11.26 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Val20-Pro698

Catalog #TFN-HM101

Size

100UG

500UG

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Human Transferrin Protein (TFN-HM101) Summary

Product Description	Recombinant Human Transferrin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val20-Pro698.
Product Background	Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human Transferrin Protein (TFN-HM101) Specifications/Details

Protein	Transferrin
Synonyms	Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1
Accession	AAH59367
Species	Human
Biotinylated	no
Amino Acid Range	Val20-Pro698
Molecular Weight	The protein has a predicted MW of 76.3 kDa. Due to glycosylation, the protein migrates to 78-82 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 0.50ug/ml determined by ELISA (QC Test). The affinity constant of 11.26 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 11.26 nM as determined in SPR assay (Biacore T200).
	Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 0.50ug/ml determined by ELISA (QC Test).
	Human Transferrin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Transferrin is greater than 95% as determined by SEC-HPLC.