Human Transferrin Protein (TFN-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val20-Pro698

Catalog: TFN-HM101

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Product Description Recombinant Human Transferrin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val20-Pro698.

Product Background

Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.

Product Category

Recombinant Protein

Target

Transferrin
Synonyms Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1

Gene/Protein ID

AAH59367

Species

Human

Exact Sequence

Val20-Pro698
Molecular Weight The protein has a predicted MW of 76.3 kDa. Due to glycosylation, the protein migrates to 78-82 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 27.50 nM as determined in SPR assay (Biacore T200).
The purity of Human Transferrin is greater than 95% as determined by SEC-HPLC.
Human Transferrin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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