Human Transferrin Protein (TFN-HM101)
Human Transferrin Protein (TFN-HM101)
Human Transferrin Protein (TFN-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 0.50ug/ml determined by ELISA (QC Test). The affinity constant of 11.26 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val20-Pro698
Catalog #TFN-HM101
Regular price $150.00
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Product Description
Recombinant Human Transferrin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val20-Pro698.
Product Background
Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.
Product Category
Recombinant Protein
Target
Transferrin
Synonyms
Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Val20-Pro698
Molecular Weight
The protein has a predicted MW of 76.3 kDa. Due to glycosylation, the protein migrates to 78-82 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 0.50ug/ml determined by ELISA (QC Test). The affinity constant of 11.26 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 11.26 nM as determined in SPR assay (Biacore T200).
Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 0.50ug/ml determined by ELISA (QC Test).
Human Transferrin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Transferrin is greater than 95% as determined by SEC-HPLC.

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