• Express System: HEK293
  • Product Tag: N-Llama IgG2b Fc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human Transferrin R Apical Domain, Llama IgG2b Fc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
  • Amino Acid Range: Lys189-Glu383
Catalog #TFR-HM6AD
Price
Regular price $34500
Regular price Sale price $34500
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Human Transferrin R/CD71 Apical Domain Protein (TFR-HM6AD) Summary

Product Description

 Recombinant Human Transferrin R/CD71 Apical Domain Protein is expressed from HEK293 with Llama IgG2b Fc tag at the N-terminus.It contains Lys189-Glu383.

Product Background

 The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Human Transferrin R/CD71 Apical Domain Protein (TFR-HM6AD) Specifications/Details

Protein
 Transferrin R/CD71
Synonyms
 TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys189-Glu383
Molecular Weight
 The protein has a predicted MW of 48.42 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Transferrin R Apical Domain, Llama IgG2b Fc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
Product Tag
 N-Llama IgG2b Fc
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Transferrin R Apical Domain, Llama IgG2b Fc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. Immobilized Human Transferrin R Apical Domain, Llama IgG2b Fc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
Human Transferrin R Apical Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Transferrin R Apical Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Transferrin R Apical Domain is greater than 90% as determined by SEC-HPLC. The purity of Human Transferrin R Apical Domain is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...