• Express System: HEK293
  • Product Tag: N-Twin Strep-His
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Transferrin R Nanodisc, Twin Strep-His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 0.89 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: MET1-Phe760
Catalog #TFR-HM60N
Price
Regular price $1,44400
Regular price Sale price $1,44400
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Size
Avantor VWR Fisher Scientific

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Human Transferrin R/CD71 Nanodisc (TFR-HM60N) Summary

Product Description

 Recombinant Human Transferrin R/CD71 Nanodisc is expressed from HEK293 with Twin Strep tag and His tag at the N-terminus.It contains MET1-Phe760.

Product Background

 The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Human Transferrin R/CD71 Nanodisc (TFR-HM60N) Specifications/Details

Protein
 Transferrin R/CD71
Synonyms
 TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 MET1-Phe760
Molecular Weight
 The protein has a predicted MW of 89.62 kDa. Due to glycosylation, the protein migrates to 93-115 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Transferrin R Nanodisc, Twin Strep-His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 0.89 nM as determined in SPR assay (Biacore T200).
Product Tag
 N-Twin Strep-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Anti-Transferrin R Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human Transferrin R Nanodisc, Twin Strep, His Tag with an affinity constant of 0.89 nM as determined in SPR assay (Biacore T200). Anti-Transferrin R Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human Transferrin R Nanodisc, Twin Strep, His Tag with an affinity constant of 0.89 nM as determined in SPR assay (Biacore T200).
Immobilized Human Transferrin R Nanodisc, Twin Strep-His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. Immobilized Human Transferrin R Nanodisc, Twin Strep-His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Immobilized Human Transferrin R Nanodisc, Twin Strep-His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Transferrin, hFc Tag with the EC50 of 70.1ng/ml determined by ELISA. Immobilized Human Transferrin R Nanodisc, Twin Strep-His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Transferrin, hFc Tag with the EC50 of 70.1ng/ml determined by ELISA.
Human Transferrin R Nanodisc on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Human Transferrin R Nanodisc on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Other Available Proteins:
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