Human TREM1 (21-136) Protein (TRM-HM10D)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.96 µM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala21-Gly136 (C69S)
Catalog #TRM-HM10D
Regular price $390.00
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Size
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Human TREM1 (21-136) Protein (TRM-HM10D) Summary

Product Description

Recombinant Human TREM1 (21-136) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala21-Gly136 (C69S).

Product Background

TREM1 (Triggering Receptor Expressed on Myeloid Cells 1) is a pro-inflammatory receptor expressed by phagocytes, which can also be released as a soluble molecule (sTREM1). The roles of TREM1 and sTREM1 in liver infection and inflammation are not clear.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human TREM1 (21-136) Protein (TRM-HM10D) Specifications/Details

Protein
TREM1
Synonyms
CD354; TREM-1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala21-Gly136 (C69S)
Molecular Weight
The protein has a predicted MW of 15.04 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 0.96 µM as determined in SPR assay (Biacore T200).
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human PGLYRP1, hFc Tag (Cat. PGL-HM201) captured on CM5 Chip via Protein A can bind Human TREM1 (21-136), His Tag with an affinity constant of 0.96 µM as determined in SPR assay (Biacore T200). Human PGLYRP1, hFc Tag (Cat. PGL-HM201) captured on CM5 Chip via Protein A can bind Human TREM1 (21-136), His Tag with an affinity constant of 0.96 µM as determined in SPR assay (Biacore T200).
Human TREM1 (21-136) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TREM1 (21-136) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human TREM1 (21-136), His Tag on Anti-His-Biosensor can bind Human PGLYRP1, hFc Tag (Cat. PGL-HM201) with an affinity constant of 42.00 nM as determined in BLI assay Loaded Human TREM1 (21-136), His Tag on Anti-His-Biosensor can bind Human PGLYRP1, hFc Tag (Cat. PGL-HM201) with an affinity constant of 42.00 nM as determined in BLI assay
The purity of Human TREM1 (21-136) is greater than 95% as determined by SEC-HPLC. The purity of Human TREM1 (21-136) is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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