- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: The affinity constant of 0.96 µM as determined in SPR assay (Biacore T200).
- Amino Acid Range: Ala21-Gly136 (C69S)
Product Description |
Recombinant Human TREM1 (21-136) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala21-Gly136 (C69S). |
Product Background |
TREM1 (Triggering Receptor Expressed on Myeloid Cells 1) is a pro-inflammatory receptor expressed by phagocytes, which can also be released as a soluble molecule (sTREM1). The roles of TREM1 and sTREM1 in liver infection and inflammation are not clear. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Protein |
TREM1 |
Synonyms |
CD354; TREM-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala21-Gly136 (C69S) |
Molecular Weight |
The protein has a predicted MW of 15.04 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.96 µM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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