Human TREM2 Protein (TRM-HM102)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human TREM2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.41 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: His19-Ser174

Catalog: TRM-HM102

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human TREM2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His19-Ser174.
Product Background	TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2- consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.
Product Category	Recombinant Protein

Target	TREM2
Synonyms	PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c
Gene/Protein ID	Q9NZC2-1
Species	Human
Exact Sequence	His19-Ser174
Molecular Weight	The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TREM2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.41 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human TREM2, His Tag captured on CM5 Chip via anti-his antibody can bind Human APOE3, hFc Tag with an affinity constant of 0.41 uM as determined in SPR assay (Biacore T200).
	Immobilized Human TREM2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test).
	Human TREM2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.