Human TREM2 Protein (TRM-HM102)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human TREM2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). The affinity constant of 20.24 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: His19-Ser174

Catalog: TRM-HM102

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Product Description Recombinant Human TREM2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His19-Ser174.

Product Background

TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2- consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.

Product Category

Recombinant Protein

Target

TREM2
Synonyms PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c
Accession

Species

Human

Amino Acid Range

His19-Ser174
Molecular Weight The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human TREM2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). The affinity constant of 20.24 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human APOE3, hFc Tag captured on CM5 Chip via Protein A can bind Human TREM2, His Tag with an affinity constant of 20.24 uM as determined in SPR assay (Biacore T200).
Immobilized Human TREM2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test).
Human TREM2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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