Human TREM2 Protein (TRM-HM102)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human TREM2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-TREM-2 Antibody, hFc Tag with the EC50 of 28.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.41 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: His19-Ser174

Catalog: TRM-HM102

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Product Description Recombinant Human TREM2 Protein is expressed from HEK293 with His tag at the C-terminal.It contains His19-Ser174.
Product Category Recombinant Protein
Target TREM2
Synonyms PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c
Gene/Protein ID Q9NZC2-1
Species Human
Exact Sequence His19-Ser174
Molecular Weight The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TREM2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-TREM-2 Antibody, hFc Tag with the EC50 of 28.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.41 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TREM2, His Tag captured on CM5 Chip via anti-his antibody can bind Human APOE3, hFc Tag with an affinity constant of 0.41 μM as determined in SPR assay (Biacore T200).
Immobilized Human TREM2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-TREM-2 Antibody, hFc Tag with the EC50 of 28.6ng/ml determined by ELISA (QC Test).
Human TREM2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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