Product Description |
Recombinant Human TREM2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His19-Ser174. |
Product Background |
TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2- consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia. |
Product Category |
Recombinant Protein |
Target |
TREM2 |
Synonyms |
PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His19-Ser174 |
Molecular Weight |
The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TREM2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). The affinity constant of 20.24 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human APOE3, hFc Tag captured on CM5 Chip via Protein A can bind Human TREM2, His Tag with an affinity constant of 20.24 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human TREM2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). |
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Human TREM2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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