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Human TrkA Protein, Ultra Low Endotoxin (TRK-HM10A-UL) Summary
Product Description |
Recombinant Human TrkA-Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala33-Gly417. |
Product Background |
TrkA, a tyrosine kinase receptor, is an essential component of the nerve growth factor (NGF) response pathway. The binding of NGF to the receptor induces receptor autophosphorylation and activation of intracellular signaling pathways, resulting in diverse biological effects. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human TrkA Protein, Ultra Low Endotoxin (TRK-HM10A-UL) Specifications/Details
Protein |
TrkA |
Synonyms |
Trk-A; NTRK1; MTC; TRK; TRKA; TrkA-I; TrkA I; TrkAI |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala33-Gly417 |
Molecular Weight |
The protein has a predicted MW of 43.00 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Beta-NGF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkA, His Tag with the EC50 of 0.21ug/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human Beta-NGF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkA, His Tag with the EC50 of 0.21ug/ml determined by ELISA. |
|
|
Human TrkA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human TrkA is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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