Product Description |
Recombinant Human TrkB/NTRK2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys32-His430. |
Product Background |
Neuron differentiation is likely regulated by a combination of transcription and growth factors. Embryonically, most geniculate neuron development is regulated by the growth factor brain derived neurotrophic factor (BDNF). BDNF expression becomes restricted to subpopulations of taste receptor cells with specific functions,the receptor for BDNF, tropomyosin kinase B receptor (TrkB), may also become developmentally restricted to a subset of taste neurons and could be one factor that is differentially expressed across taste neuron subsets. |
Product Category |
Recombinant Protein |
Target |
TrkB/NTRK2 |
Synonyms |
GP145-TrkB; NTRK2; TRKB |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys32-His430 |
Molecular Weight |
The protein has a predicted MW of 45.2 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39ug/ml determined by ELISA (QC Test). |
|
|
Human TrkB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human TrkB is greater than 95% as determined by SEC-HPLC. |
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