Human TrkB/NTRK2 Protein (TKB-HM101)
Human TrkB/NTRK2 Protein (TKB-HM101)
Human TrkB/NTRK2 Protein (TKB-HM101)
Human TrkB/NTRK2 Protein (TKB-HM101)
Human TrkB/NTRK2 Protein (TKB-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Cys32-His430
Catalog #TKB-HM101
Regular price $225.00
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Product Description
Recombinant Human TrkB/NTRK2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys32-His430.
Product Background
Neuron differentiation is likely regulated by a combination of transcription and growth factors. Embryonically, most geniculate neuron development is regulated by the growth factor brain derived neurotrophic factor (BDNF). BDNF expression becomes restricted to subpopulations of taste receptor cells with specific functions,the receptor for BDNF, tropomyosin kinase B receptor (TrkB), may also become developmentally restricted to a subset of taste neurons and could be one factor that is differentially expressed across taste neuron subsets.
Product Category
Recombinant Protein
Target
TrkB/NTRK2
Synonyms
GP145-TrkB; NTRK2; TRKB
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Cys32-His430
Molecular Weight
The protein has a predicted MW of 45.2 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39ug/ml determined by ELISA (QC Test).
Human TrkB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TrkB is greater than 95% as determined by SEC-HPLC.

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