SEC-HPLC chromatogram showing a single major peak at 7.859 min, confirming the purity of the protein product.
Human TrkB/NTRK2 Protein (TKB-HM101)
Human TrkB/NTRK2 Protein (TKB-HM101)
Human TrkB/NTRK2 Protein (TKB-HM101)
Human TrkB/NTRK2 Protein (TKB-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Cys32-His430
Catalog #TKB-HM101
Regular price $247.50
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Human TrkB/NTRK2 Protein (TKB-HM101) Summary

Product Description

Recombinant Human TrkB/NTRK2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys32-His430.

Product Background

Neuron differentiation is likely regulated by a combination of transcription and growth factors. Embryonically, most geniculate neuron development is regulated by the growth factor brain derived neurotrophic factor (BDNF). BDNF expression becomes restricted to subpopulations of taste receptor cells with specific functions,the receptor for BDNF, tropomyosin kinase B receptor (TrkB), may also become developmentally restricted to a subset of taste neurons and could be one factor that is differentially expressed across taste neuron subsets.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human TrkB/NTRK2 Protein (TKB-HM101) Specifications/Details

Protein
TrkB/NTRK2
Synonyms
GP145-TrkB; NTRK2; TRKB
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Cys32-His430
Molecular Weight
The protein has a predicted MW of 45.2 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39ug/ml determined by ELISA (QC Test). Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39ug/ml determined by ELISA (QC Test).
Human TrkB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TrkB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TrkB is greater than 95% as determined by SEC-HPLC. The purity of Human TrkB is greater than 95% as determined by SEC-HPLC.
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