Human TrkB/NTRK2 Protein (TKB-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 50.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Cys32-His430

Catalog: TKB-HM101

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Product Description	Recombinant Human TrkB/NTRK2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys32-His430.
Product Background	Neuron differentiation is likely regulated by a combination of transcription and growth factors. Embryonically, most geniculate neuron development is regulated by the growth factor brain derived neurotrophic factor (BDNF). BDNF expression becomes restricted to subpopulations of taste receptor cells with specific functions,the receptor for BDNF, tropomyosin kinase B receptor (TrkB), may also become developmentally restricted to a subset of taste neurons and could be one factor that is differentially expressed across taste neuron subsets.
Product Category	Recombinant Protein

Target	TrkB/NTRK2
Synonyms	GP145-TrkB; NTRK2; TRKB
Gene/Protein ID	AAH31835
Species	Human
Exact Sequence	Cys32-His430
Molecular Weight	The protein has a predicted MW of 45.2 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 50.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 50.5ng/ml determined by ELISA.
	The purity of Human TrkB is greater than 95% as determined by SEC-HPLC.
	Human TrkB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.