- Express System: HEK293
- Product Tag: C-hFc (IgG1)
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human TSLP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human TSLPR, His Tag with the EC50 of 0.44 ug/ml determined by ELISA (QC Test).
- Amino Acid Range: Tyr29-Gln159
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Human TSLP Protein (TSP-HM201) Summary
Product Description |
Recombinant Human TSLP Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Tyr29-Gln159. |
Product Background |
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human TSLP Protein (TSP-HM201) Specifications/Details
Protein |
TSLP |
Synonyms |
thymic stromal lymphopoietin; TSLP |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr29-Gln159 |
Molecular Weight |
The protein has a predicted MW of 41.69 kDa. Due to furin cleavage site, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TSLP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human TSLPR, His Tag with the EC50 of 0.44 ug/ml determined by ELISA (QC Test). |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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