Human TSLP Protein (TSP-HM201)
Human TSLP Protein (TSP-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human TSLP, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TSLPR, His Tag with the EC50 of 1.33 ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Tyr29-Gln159
Catalog #TSP-HM201
Regular price $315.00
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Product Description

Recombinant Human TSLP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Tyr29-Gln159.

Product Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Product Category

Recombinant Protein / Antibody discovery

Protein

TSLP

Synonyms

thymic stromal lymphopoietin; TSLP

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Tyr29-Gln159

Molecular Weight

The protein has a predicted MW of 41.69 kDa. Due to furin cleavage site, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human TSLP, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TSLPR, His Tag with the EC50 of 1.33 ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human TSLP, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TSLPR, His Tag with the EC50 of 1.33 ug/ml determined by ELISA (QC Test). Immobilized Human TSLP, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TSLPR, His Tag with the EC50 of 1.33 ug/ml determined by ELISA (QC Test).
Immobilized Human TSLP, hFc Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-TSLP Antibody, hFc Tag with the EC50 of 87.0 ng/ml determined by ELISA. Immobilized Human TSLP, hFc Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-TSLP Antibody, hFc Tag with the EC50 of 87.0 ng/ml determined by ELISA.
Human TSLP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TSLP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TSLP is greater than 95% as determined by SEC-HPLC. The purity of Human TSLP is greater than 95% as determined by SEC-HPLC.

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