Name: Human TSLP (R127A, R130A) Protein (TSP-HM202)
Brand: KactusBio
SKU: TSP-HM202-100UG
Price: 347.0 USD
Availability: InStock

Human TSLP (R127A, R130A) Protein (TSP-HM202)

Immobilized Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.

Immobilized Human TSLP (R127A, R130A), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.

Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 12.70 nM as determined in BLI assay (Gator® Prime).

Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 0.41 nM as determined in BLI assay (Gator® Prime).

The purity of Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Amino Acid Range: Tyr29-Gln159 (R127A, R130A)

Catalog #TSP-HM202

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human TSLP (R127A, R130A) Protein (TSP-HM202) Summary

Product Description	Recombinant Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr29-Gln159 (R127A, R130A).
Product Background	Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human TSLP (R127A, R130A) Protein (TSP-HM202) Specifications/Details

Protein	TSLP
Synonyms	thymic stromal lymphopoietin; TSLP
Accession	Q969D9-1
Species	Human
Biotinylated	no
Amino Acid Range	Tyr29-Gln159 (R127A, R130A)
Molecular Weight	The protein has a predicted MW of 16.37 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
	Immobilized Human TSLP (R127A, R130A), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
	Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 12.70 nM as determined in BLI assay (Gator® Prime).
	Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 0.41 nM as determined in BLI assay (Gator® Prime).
	The purity of Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.