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Human TSLP (R127A, R130A) Protein (TSP-HM202) Summary
Product Description |
Recombinant Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr29-Gln159 (R127A, R130A). |
Product Background |
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human TSLP (R127A, R130A) Protein (TSP-HM202) Specifications/Details
Protein |
TSLP |
Synonyms |
thymic stromal lymphopoietin; TSLP |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr29-Gln159 (R127A, R130A) |
Molecular Weight |
The protein has a predicted MW of 16.37 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. |
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Immobilized Human TSLP (R127A, R130A), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. |
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Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 12.70 nM as determined in BLI assay. |
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Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 0.41 nM as determined in BLI assay. |
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The purity of Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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