Human TSLPR Protein (TSP-HM10R)
Human TSLPR Protein (TSP-HM10R)
Human TSLPR Protein (TSP-HM10R)
Human TSLPR Protein (TSP-HM10R)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TSLPR, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Lys231
Catalog #TSP-HM10R
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Product Description
 Recombinant Human TSLPR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly25-Lys231.
Product Background
 TSLP is an epithelial cell-derived cytokine synthesized in response to various stimuli, including protease allergens and microorganisms like viruses and bacteria. Biological functions of TSLP require heterodimer formation between the TSLP receptor (TSLPR) and IL-7 receptor-α, which polarize dendritic cells to induce type 2 inflammation and directly expand and/or activate Th2 cells, group 2 innate lymphoid cells, basophils, and other immune cells.
Product Category
 Recombinant Protein
Target
 TSLPR
Synonyms
 TSLP receptor; TSLPR; CRL2; CRLF2Y; CRLF2; IL-XR; p2RY8/CRLF2 fusion; CRLF2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Lys231
Molecular Weight
 The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TSLPR, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TSLPR, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
Immobilized Human TSLP, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, His Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test).
Human TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC.
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