Name: Human TSLPR Protein (TSP-HM20R)
Brand: KactusBio
SKU: TSP-HM20R-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TSLP (R127A, R130A), His Tag at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). The affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly25-Lys231
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Catalog #TSP-HM20R

Size

100UG

500UG

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Human TSLPR Protein (TSP-HM20R) Summary

Product Description	Recombinant Human TSLPR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly25-Lys231.
Product Background	Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human TSLPR Protein (TSP-HM20R) Specifications/Details

Protein	TSLPR
Synonyms	TSLP receptor; TSLPR; CRL2; CRLF2Y; CRLF2; IL-XR; p2RY8/CRLF2 fusion; CRLF2
Accession	Q9HC73-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Lys231
Molecular Weight	The protein has a predicted MW of 50.8 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TSLP (R127A, R130A), His Tag at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). The affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human TSLP, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human TSLPR, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TSLP (R127A, R130A), His Tag at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
	Immobilized Human TSLPR, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSLPR Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA.
	Human TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP, His Tag with an affinity constant of 31.30 nM as determined in BLI assay (Gator® Prime).
	The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC.