Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
Human TSLPR Protein (TSP-HM20R)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TSLP (R127A, R130A), His Tag at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). The affinity constant of 1.04 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly25-Lys231
Catalog #TSP-HM20R
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Product Description
 Recombinant Human TSLPR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly25-Lys231.
Product Background
 Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2).
Product Category
 Recombinant Protein
Target
 TSLPR
Synonyms
 TSLP receptor; TSLPR; CRL2; CRLF2Y; CRLF2; IL-XR; p2RY8/CRLF2 fusion; CRLF2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Lys231
Molecular Weight
 The protein has a predicted MW of 50.8 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TSLP (R127A, R130A), His Tag at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). The affinity constant of 1.04 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TSLP, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human TSLPR, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).
Immobilized Human TSLP (R127A, R130A), His Tag at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Immobilized Human TSLPR, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSLPR Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA.
Human TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC.
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