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Human TSLPR Protein, Ultra Low Endotoxin (TSP-HM20R-UL) Summary
Product Description |
Recombinant Human TSLPR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly25-Lys231. |
Product Background |
Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human TSLPR Protein, Ultra Low Endotoxin (TSP-HM20R-UL) Specifications/Details
Protein |
TSLPR |
Synonyms |
TSLP receptor; TSLPR; CRL2; CRLF2Y; CRLF2; IL-XR; p2RY8/CRLF2 fusion; CRLF2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Lys231 |
Molecular Weight |
The protein has a predicted MW of 50.8 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TSLP, His Tag (Cat. TSP-HM101) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 27.6ng/ml determined by ELISA. The affinity constant of 1.04 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human TSLP, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human TSLPR, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human TSLP, His Tag (Cat. TSP-HM101) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 27.6ng/ml determined by ELISA. |
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Immobilized Human TSLP (R127A, R130A), His Tag (Cat. TSP-HM202) at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. |
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Immobilized Human TSLPR, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSLPR Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA. |
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Human TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP, His Tag with an affinity constant of 31.30 nM as determined in BLI assay . |
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The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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