Human ULBP-1 Protein (ULB-HM4P1)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ULBP-1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 33.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly26-Pro215

Catalog: ULB-HM4P1

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Product Description	Recombinant Human ULBP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Pro215.
Product Background	Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells.
Product Category	Recombinant Protein

Target	ULBP-1
Synonyms	ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; MULT-1; NKG2DL; AET1I; ULBP1; ULBP-1
Gene/Protein ID	Q9BZM6
Species	Human
Exact Sequence	Gly26-Pro215
Molecular Weight	The protein has a predicted MW of 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human ULBP-1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 33.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PB, 0.5M NaCl, 0.1M L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human ULBP-1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 33.2ng/ml determined by ELISA.
	The purity of Human ULBP-1 is greater than 95% as determined by SEC-HPLC.
	Human ULBP-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.