Human ULBP-1 Protein (ULB-HM4P1)
Human ULBP-1 Protein (ULB-HM4P1)
Human ULBP-1 Protein (ULB-HM4P1)
Human ULBP-1 Protein (ULB-HM4P1)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ULBP-1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly26-Pro215
Catalog #ULB-HM4P1
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Product Description
 Recombinant Human ULBP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Pro215.
Product Background
 Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells.
Product Category
 Recombinant Protein
Target
 ULBP-1
Synonyms
 ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; MULT-1; NKG2DL; AET1I; ULBP1; ULBP-1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly26-Pro215
Molecular Weight
 The protein has a predicted MW of 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ULBP-1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM PB, 0.5M NaCl, 0.1M L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ULBP-1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA.
Human ULBP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ULBP-1 is greater than 95% as determined by SEC-HPLC.
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