Product Description |
Recombinant Human ULBP-4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains His31-Asp225. |
Product Background |
UL16-binding proteins (ULBPs) are markers of cellular stress which are upregulated on the surface of virus-infected and tumor cells. ULBPs are expressed by a variety of leukemias, carcinomas, melanomas, and tumor cell lines.ULBP4 is associated with higher levels of CD8+/NKG2D+ lymphocytes infiltrating the carcinoma and is a prognosticator of improved survival. |
Product Category |
Recombinant Protein |
Protein |
ULBP-4 |
Synonyms |
N2DL-4; NKG2DL4; RAE-1-like transcript 4; RAET1E; LETAL; N2DL4; ULBP4; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His31-Asp225 |
Molecular Weight |
The protein has a predicted MW of 49.12 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human ULBP-4, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). |
|
|
Human ULBP-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human ULBP-4 is greater than 95% as determined by SEC-HPLC. |
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