Human UltraPro Mutant CD19 Protein (CD1-HM129)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
  • Amino Acid Range: Pro20-Pro278 with site mutations
Catalog #CD1-HM129
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Size
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Human UltraPro Mutant CD19 Protein (CD1-HM129) Summary

Product Description

 Recombinant Human UltraPro Mutant CD19 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro20-Pro278 with site mutations.

Product Background

 CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets

Human UltraPro Mutant CD19 Protein (CD1-HM129) Specifications/Details

Protein
 CD19
Synonyms
 CD19; B4; CVID3; Leu-12; MGC12802
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro20-Pro278 with site mutations
Molecular Weight
 The protein has a predicted MW of 29.93 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 9.5ng/ml determined by ELISA. Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 9.5ng/ml determined by ELISA.
Immobilized Human UltraPro Mutant CD19, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. Immobilized Human UltraPro Mutant CD19, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
Immobilized Human UltraPro Mutant CD19, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. Immobilized Human UltraPro Mutant CD19, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Human UltraPro Mutant CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human UltraPro Mutant CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Coltuximab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 9.48 nM as determined in BLI assay. Loaded Coltuximab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 9.48 nM as determined in BLI assay.
Loaded FMC63 on MFC-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 3.80 nM as determined in BLI assay. Loaded FMC63 on MFC-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 3.80 nM as determined in BLI assay.
Loaded Inebilizumab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 3.97 nM as determined in BLI assay. Loaded Inebilizumab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 3.97 nM as determined in BLI assay.
Loaded Denintuzumab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 8.05 nM as determined in BLI assay. Loaded Denintuzumab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 8.05 nM as determined in BLI assay.
The purity of Human UltraPro Mutant CD19 is greater than 95% as determined by SEC-HPLC. The purity of Human UltraPro Mutant CD19 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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