Human UltraPro Mutant CD19 Protein, Ultra Low Endotoxin (CD1-HM129-UL)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Amino Acid Range: Pro20-Pro278 with site mutations

Catalog #CD1-HM129-UL

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$264.00

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Human UltraPro Mutant CD19 Protein, Ultra Low Endotoxin (CD1-HM129-UL) Summary

Product Description	Recombinant Human UltraPro Mutant CD19 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro20-Pro278 with site mutations.
Product Background	CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets

Protein	CD19
Synonyms	CD19; B4; CVID3; Leu-12; MGC12802
Accession	P15391-1
Species	Human
Biotinylated	no
Amino Acid Range	Pro20-Pro278 with site mutations
Molecular Weight	The protein has a predicted MW of 29.93 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
	Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 9.5ng/ml determined by ELISA.
	Immobilized Human UltraPro Mutant CD19, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
	Immobilized Human UltraPro Mutant CD19, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
	Human UltraPro Mutant CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Coltuximab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 9.48 nM as determined in BLI assay.
	Loaded FMC63 on MFC-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 3.80 nM as determined in BLI assay.
	Loaded Inebilizumab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 3.97 nM as determined in BLI assay.
	Loaded Denintuzumab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 8.05 nM as determined in BLI assay.
	The purity of Human UltraPro Mutant CD19 is greater than 95% as determined by SEC-HPLC.

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