Human VAP-1 Protein (VAP-HM101) Summary
Product Description |
Recombinant Human VAP-1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gly27-Asn763. |
Product Background |
The semicarbazide-sensitive amine oxidase (SSAO), also known as vascular adhesion protein-1 (VAP-1) or primary amine oxidase (PrAO), is a deaminating enzyme highly expressed in vessels that generates harmful products as a result of its enzymatic activity. As a multifunctional enzyme, it is also involved in inflammation through its ability to bind and promote the transmigration of circulating leukocytes into inflamed tissues. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human VAP-1 Protein (VAP-HM101) Specifications/Details
Protein |
VAP-1 |
Synonyms |
HPAO; SSAO; VAP-1; VAP 1; AOC3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly27-Asn763 |
Molecular Weight |
The protein has a predicted MW of 82.88 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human VAP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human VAP-1 is greater than 95% as determined by SEC-HPLC. |
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