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Non-biotinylated Human VEGF-C/Flt4-L Protein, Ultra Low Endotoxin (VEG-HM4F1-UL) Summary
Product Description |
Recombinant Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr103-Arg227. |
Product Background |
the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human VEGF-C/Flt4-L Protein, Ultra Low Endotoxin (VEG-HM4F1-UL) Specifications/Details
Protein |
VEGF-C/Flt4-L |
Synonyms |
VEGFC; VEGFc; Flt4-L; VRP; VEGF-C |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr103-Arg227 |
Molecular Weight |
The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 50mM MES (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human VEGF R3, His Tag immobilized on CM5 Chip can bind Human VEGF-C, His Tag with an affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test). |
|
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Human VEGF-C on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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