Human VEGF-C/Flt4-L Protein (VEG-HM4F1)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr103-Arg227

Catalog: VEG-HM4F1

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Product Description Recombinant Human VEGF-C/Flt4-L Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Thr103-Arg227.
Product Category Recombinant Protein
Target VEGF-C/Flt4-L
Synonyms VEGFC; VEGFc; Flt4-L; VRP; VEGF-C
Gene/Protein ID Q6FH59
Species Human
Exact Sequence Thr103-Arg227
Molecular Weight The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCI (pH 6.0).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human VEGF R3, His Tag immobilized on CM5 Chip can bind Human VEGF-C, His Tag with an affinity constant of 0.29 nM as determined in SPR assay (Biacore T200).
Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA.
The purity of Human VEGF-C is greater than 95% as determined by SEC-HPLC.
Human VEGF-C on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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