Human VEGF-D Protein (VEG-HM10D)

Immobilized Human VEGF R3, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-D, His Tag with the EC50 of 2.06ug/ml determined by ELISA (QC Test).

Human VEGF-D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human VEGF-D is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human VEGF R3, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-D, His Tag with the EC50 of 2.06ug/ml determined by ELISA (QC Test).
Amino Acid Range: Phe89-Arg205

Catalog #VEG-HM10D

Price

$297⁰⁰

Size

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Human VEGF-D Protein (VEG-HM10D) Summary

Product Description

Recombinant Human VEGF-D Protein is expressed from HEK293 with His tag at the C-terminus.It contains Phe89-Arg205.

Product Background

Vascular endothelial growth factor-D (VEGF-D) is a secreted glycoprotein that can activate VEGF receptors on the endothelium, is a mitogen for endothelial cells and promotes the growth and remodeling of blood vessels and lymphatic vessels. VEGF-D, as with other members of the VEGF family, falls within a structural superfamily of growth factors containing a cystine knot motif, which involves a highly distinctive clustered arrangement of three intrasubunit cystine bridges.

Human VEGF-D Protein (VEG-HM10D) Specifications/Details

Protein	VEGF-D
Synonyms	FIGF; VEGFD;
Accession	O43915
Species	Human
Biotinylated	no
Amino Acid Range	Phe89-Arg205
Molecular Weight	The protein has a predicted MW of 14.72 kDa. Due to glycosylation, the protein migrates to 23-43 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human VEGF R3, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-D, His Tag with the EC50 of 2.06ug/ml determined by ELISA (QC Test).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human VEGF R3, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-D, His Tag with the EC50 of 2.06ug/ml determined by ELISA (QC Test).
	Human VEGF-D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human VEGF-D is greater than 95% as determined by SEC-HPLC.