Human VEGF R1/FLT-1 Protein (VGF-HM4R1)
Human VEGF R1/FLT-1 Protein (VGF-HM4R1)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser27-Asn756
Catalog #VGF-HM4R1
Regular price $270.00
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Product Description
Recombinant Human VEGF R1/FLT-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser27-Asn756.
Product Background
VEGFR1 (vascular endothelial growth factor receptor 1), also called Flt-1 (Fms-like tyrosine kinase), is a 180 kDa type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTKs).yrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells.
Product Category
Recombinant Protein
Target
VEGF R1/FLT-1
Synonyms
FLT; FLT1; Flt-1; FRT; VEGF R1; VEGFR1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser27-Asn756
Molecular Weight
The protein has a predicted MW of 85.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA.
Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 16.5ng/ml determined by ELISA (QC Test).
Human VEGF R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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