Human VEGF R2/KDR Protein (VGF-HM1R2)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF165, No Tag (Cat. VEG-HM065) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, HisTag with the EC50 of 0.35ug/ml determined by ELISA.
  • Amino Acid Range: Ala20-Glu764
Catalog #VGF-HM1R2
Regular price $240.00
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Human VEGF R2/KDR Protein (VGF-HM1R2) Summary

Product Description

Recombinant Human VEGF R2/KDR Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala20-Glu764.

Product Background

The vascular endothelial growth factor receptor-2 (VEGFR-2/KDR/flk-1) functions as the primary mediator of vascular endothelial growth factor activation in endothelial cells. Regulation of VEGFR-2 expression appears critical in mitogenesis, differentiation, and angiogenesis. Transcriptional regulation of the VEGFR-2 is complex and may involve multiple putative upstream regulatory elements including E boxes.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets

Human VEGF R2/KDR Protein (VGF-HM1R2) Specifications/Details

Protein
VEGF R2/KDR
Synonyms
CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala20-Glu764
Molecular Weight
The protein has a predicted MW of 84.88 kDa. Due to glycosylation, the protein migrates to 120-160 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human VEGF165, No Tag (Cat. VEG-HM065) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, HisTag with the EC50 of 0.35ug/ml determined by ELISA.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human VEGF165, No Tag (Cat. VEG-HM065) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, HisTag with the EC50 of 0.35ug/ml determined by ELISA. Immobilized Human VEGF165, No Tag (Cat. VEG-HM065) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, HisTag with the EC50 of 0.35ug/ml determined by ELISA.
Immobilized Human VEGF R2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. Immobilized Human VEGF R2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF R2 is greater than 95% as determined by SEC-HPLC. The purity of Human VEGF R2 is greater than 95% as determined by SEC-HPLC.
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