Human VEGF R2/KDR Protein (VGF-HM3R2)
Human VEGF R2/KDR Protein (VGF-HM3R2)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF165 1ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala20-Glu764
Catalog #VGF-HM3R2
Regular price $240.00
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Product Description
Recombinant Human VEGF R2/KDR Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Glu764.
Product Background
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.
Product Category
Recombinant Protein
Target
VEGF R2/KDR
Synonyms
CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala20-Glu764
Molecular Weight
The protein has a predicted MW of 110 kDa. Due to glycosylation, the protein migrates to 150-200 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human VEGF165 1ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-mFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF165 1ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA.
Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF R2 is greater than 95% as determined by SEC-HPLC.

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