Human VEGF R2/KDR Protein (VGF-HM3R2)
Catalog: VGF-HM3R2
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| Product Description | Recombinant Human VEGF R2/KDR Protein is expressed from Expi293 with mFc (IgG1) tag at the C-terminal.It contains Ala20-Glu764. |
| Product Category | Recombinant Protein |
| Target | VEGF R2/KDR |
| Synonyms | CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73 |
| Gene/Protein ID | P35968-1 |
| Species | Human |
| Exact Sequence | Ala20-Glu764 |
| Molecular Weight | The protein has a predicted MW of 110 kDa. Due to glycosylation, the protein migrates to 150-200 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human VEGF165 1μg/ml (100μl/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-mFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human VEGF165 1μg/ml (100μl/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA. |
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The purity of Human VEGF R2 is greater than 95% as determined by SEC-HPLC. |
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Human VEGF R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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