Human VEGF R3/FLT4 Protein (VGF-HM2R3)
Catalog: VGF-HM2R3
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Product Description | Recombinant Human VEGF R3/FLT4 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Tyr25-Ile776. |
Product Category | Recombinant Protein |
Target | VEGF R3/FLT4 |
Synonyms | VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL |
Gene/Protein ID | P35916-1 |
Species | Human |
Exact Sequence | Tyr25-Ile776 |
Molecular Weight | The protein has a predicted MW of 111.3 kDa. Due to glycosylation, the protein migrates to 135-155 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-hFc |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl, 100mM Glycine (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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