Human VEGF R3/FLT4 Protein (VGF-HM2R3)
Human VEGF R3/FLT4 Protein (VGF-HM2R3)
Human VEGF R3/FLT4 Protein (VGF-HM2R3)
Human VEGF R3/FLT4 Protein (VGF-HM2R3)
Human VEGF R3/FLT4 Protein (VGF-HM2R3)
Human VEGF R3/FLT4 Protein (VGF-HM2R3)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 17.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Tyr25-Ile776
Catalog #VGF-HM2R3
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Product Description
 Recombinant Human VEGF R3/FLT4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Tyr25-Ile776.
Product Background
 Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.
Product Category
 Recombinant Protein
Target
 VEGF R3/FLT4
Synonyms
 VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Tyr25-Ile776
Molecular Weight
 The protein has a predicted MW of 111.3 kDa. Due to glycosylation, the protein migrates to 135-155 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 17.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl, 100mM Glycine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 17.9ng/ml determined by ELISA (QC Test).
Immobilized Human VEGF R3, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-D, His Tag with the EC50 of 0.44ug/ml determined by ELISA.
Human VEGF R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF R3 is greater than 95% as determined by SEC-HPLC.
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