Human VEGF R3/FLT4 Protein (VGF-HM2R3)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr25-Ile776

Catalog: VGF-HM2R3

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Product Description Recombinant Human VEGF R3/FLT4 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Tyr25-Ile776.
Product Category Recombinant Protein
Target VEGF R3/FLT4
Synonyms VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL
Gene/Protein ID P35916-1
Species Human
Exact Sequence Tyr25-Ile776
Molecular Weight The protein has a predicted MW of 111.3 kDa. Due to glycosylation, the protein migrates to 135-155 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl, 100mM Glycine (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA.
The purity of Human VEGF R3/FLT4 is greater than 95% as determined by SEC-HPLC.
Human VEGF R3/FLT4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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