Human VEGF R3/FLT4 Protein (VGF-HM4R3)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Tyr25-Ile776

Catalog: VGF-HM4R3

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Product Description	Recombinant Human VEGF R3/FLT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr25-Ile776.
Product Background	Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.
Product Category	Recombinant Protein

Target	VEGF R3/FLT4
Synonyms	VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL
Gene/Protein ID	P35916-1
Species	Human
Exact Sequence	Tyr25-Ile776
Molecular Weight	The protein has a predicted MW of 87.4 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human VEGF R3, His Tag immobilized on CM5 Chip can bind Human VEGF-C, His Tag with an affinity constant of 0.29 nM as determined in SPR assay (Biacore T200).
	The purity of Human VEGF R3 is greater than 95% as determined by SEC-HPLC.
	Human VEGF R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.