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Non-biotinylated Human VEGF R3/FLT4 Protein (VGF-HM4R3) Summary
Product Description |
Recombinant Human VEGF R3/FLT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr25-Ile776. |
Product Background |
Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Non-biotinylated Human VEGF R3/FLT4 Protein (VGF-HM4R3) Specifications/Details
Protein |
VEGF R3/FLT4 |
Synonyms |
VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr25-Ile776 |
Molecular Weight |
The protein has a predicted MW of 87.4 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human VEGF R3, His Tag immobilized on CM5 Chip can bind Human VEGF-C, His Tag with an affinity constant of 0.29 nM as determined in SPR assay (Biacore T200). |
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Human VEGF R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human VEGF R3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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