Product Description |
Recombinant Human VEGF110 Protein is expressed from HEK293 without tag.It contains Ala27-Arg136. |
Product Background |
Vascular endothelial growth factor-A (VEGF-A) is a critical angiogenic factor which is mainly secreted from podocytes and epithelial cells in kidney and plays an important role in renal pathophysiology. In recent years, functions of different isoforms of VEGF-A and the new secretion approach via extracellular vesicles (EVs) have been identified. |
Product Category |
Recombinant Protein |
Target |
VEGF110 |
Synonyms |
VEGF; VEGFA; L-VEGF; N-VEGF; VPF; MVCD1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala27-Arg136 |
Molecular Weight |
The protein has a predicted MW of 12.70 kDa. Due to glycosylation, the protein migrates to 13 kDa and 17-23 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). |
|
|
Human VEGF110 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human VEGF110 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
