• Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ala27-Arg136
Catalog #VEG-HM010
Price
Regular price $36300
Regular price Sale price $36300
/
Size
Avantor VWR Fisher Scientific

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Human VEGF110 Protein (VEG-HM010) Summary

Product Description

 Recombinant Human VEGF110 Protein is expressed from HEK293 without tag.It contains Ala27-Arg136.

Product Background

 Vascular endothelial growth factor-A (VEGF-A) is a critical angiogenic factor which is mainly secreted from podocytes and epithelial cells in kidney and plays an important role in renal pathophysiology. In recent years, functions of different isoforms of VEGF-A and the new secretion approach via extracellular vesicles (EVs) have been identified.

Human VEGF110 Protein (VEG-HM010) Specifications/Details

Protein
 VEGF110
Synonyms
 VEGF; VEGFA; L-VEGF; N-VEGF; VPF; MVCD1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Arg136
Molecular Weight
 The protein has a predicted MW of 12.70 kDa. Due to glycosylation, the protein migrates to 13 kDa and 17-23 kDa under reduced (R) condition, and 30-40 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
Human VEGF110 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human VEGF110 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The bioactivity was measured using a luciferase reporter cell line which stably expresses the KDR receptor. The ED50 for this effect is < 8 ng/mL. The bioactivity was measured using a luciferase reporter cell line which stably expresses the KDR receptor. The ED50 for this effect is < 8 ng/mL.
The purity of Human VEGF110 is greater than 95% as determined by SEC-HPLC. The purity of Human VEGF110 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...