Name: Human VEGF110 Protein (VEG-HM010)
Brand: KactusBio
SKU: VEG-HM010-100UG
Price: 363.0 USD
Availability: InStock

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
Amino Acid Range: Ala27-Arg136

Catalog #VEG-HM010

Size

100UG

500UG

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Human VEGF110 Protein (VEG-HM010) Summary

Product Description	Recombinant Human VEGF110 Protein is expressed from HEK293 without tag.It contains Ala27-Arg136.
Product Background	Vascular endothelial growth factor-A (VEGF-A) is a critical angiogenic factor which is mainly secreted from podocytes and epithelial cells in kidney and plays an important role in renal pathophysiology. In recent years, functions of different isoforms of VEGF-A and the new secretion approach via extracellular vesicles (EVs) have been identified.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human VEGF110 Protein (VEG-HM010) Specifications/Details

Protein	VEGF110
Synonyms	VEGF; VEGFA; L-VEGF; N-VEGF; VPF; MVCD1
Accession	P15692-9
Species	Human
Biotinylated	no
Amino Acid Range	Ala27-Arg136
Molecular Weight	The protein has a predicted MW of 12.70 kDa. Due to glycosylation, the protein migrates to 13 kDa and 17-23 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
Product Tag	No Tag
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
	Human VEGF110 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL (QC Test).
	The purity of Human VEGF110 is greater than 95% as determined by SEC-HPLC.

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