Human VEGF110 Protein (VEG-HM010)
Human VEGF110 Protein (VEG-HM010)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala27-Arg136
Catalog #VEG-HM010
Regular price $330.00
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Product Description
Recombinant Human VEGF110 Protein is expressed from HEK293 without tag.It contains Ala27-Arg136.
Product Background
Vascular endothelial growth factor-A (VEGF-A) is a critical angiogenic factor which is mainly secreted from podocytes and epithelial cells in kidney and plays an important role in renal pathophysiology. In recent years, functions of different isoforms of VEGF-A and the new secretion approach via extracellular vesicles (EVs) have been identified.
Product Category
Recombinant Protein
Target
VEGF110
Synonyms
VEGF; VEGFA; L-VEGF; N-VEGF; VPF; MVCD1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala27-Arg136
Molecular Weight
The protein has a predicted MW of 12.70 kDa. Due to glycosylation, the protein migrates to 13 kDa and 17-23 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
No Tag
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
Human VEGF110 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF110 is greater than 95% as determined by SEC-HPLC.

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