- Express System: HEK293
- Product Tag: No Tag
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 59.3ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Ala27-Arg136
Product Description |
Recombinant Human VEGF110 Protein is expressed from HEK293 without tag.It contains Ala27-Arg136. |
Product Background |
Vascular endothelial growth factor-A (VEGF-A) is a critical angiogenic factor which is mainly secreted from podocytes and epithelial cells in kidney and plays an important role in renal pathophysiology. In recent years, functions of different isoforms of VEGF-A and the new secretion approach via extracellular vesicles (EVs) have been identified. |
Product Category |
Recombinant Protein |
Target |
VEGF110 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala27-Arg136 |
Molecular Weight |
The protein has a predicted MW of 12.70 kDa. Due to glycosylation, the protein migrates to 13 kDa and 17-23 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human VEGF110, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 59.3ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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