• Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 19.1ng/ml determined by ELISA.
  • Amino Acid Range: Ala27-Arg147
Catalog #VEG-HM021-UL
Price
Regular price $1,210.00
Regular price Sale price $1,210.00
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Size
Avantor VWR Fisher Scientific

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Human VEGF121 Protein, Ultra Low Endotoxin (VEG-HM021-UL) Summary

Product Description

 Recombinant Human VEGF121 Protein is expressed from HEK293 without tag.It contains Ala27-Arg147.

Product Background

 Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Human VEGF121 Protein, Ultra Low Endotoxin (VEG-HM021-UL) Specifications/Details

Protein
 VEGF121
Synonyms
 VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Arg147
Molecular Weight
 The protein has a predicted MW of 14.07 kDa. Due to glycosylation, the protein migrates to 15-17 kDa and 20-24 kDa under reduced (R) condition, and 30-40 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 19.1ng/ml determined by ELISA.
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 19.1ng/ml determined by ELISA. Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 19.1ng/ml determined by ELISA.
Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF121 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF121 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.
Human VEGF121 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human VEGF121 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL. Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL.
The purity of Human VEGF121 Protein is greater than 90% as determined by SEC-HPLC. The purity of Human VEGF121 Protein is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...