Human VEGF121 Protein (VEG-HM021)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala27-Arg147
Catalog #VEG-HM021
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Product Description
 Recombinant Human VEGF121 Protein is expressed from HEK293 without tag.It contains Ala27-Arg147.
Product Background
 Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Product Category
 Recombinant Protein
Target
 VEGF121
Synonyms
 VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Arg147
Molecular Weight
 The protein has a predicted MW of 14.07 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
 No Tag
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7ng/ml determined by ELISA.
Human VEGF121 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF121 Protein is greater than 90% as determined by SEC-HPLC.

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