Name: Human VEGF121 Protein (VEG-HM021)
Brand: KactusBio
SKU: VEG-HM021-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala27-Arg147

Catalog #VEG-HM021

Size

100UG

500UG

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Product Description	Recombinant Human VEGF121 Protein is expressed from HEK293 without tag.It contains Ala27-Arg147.
Product Background	Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Product Category	Recombinant Protein / Antibody discovery

Protein	VEGF121
Synonyms	VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
Accession	P15692-9
Species	Human
Biotinylated	no
Amino Acid Range	Ala27-Arg147
Molecular Weight	The protein has a predicted MW of 14.07 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7ng/ml determined by ELISA (QC Test).
	Human VEGF121 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL (QC Test).
	The purity of Human VEGF121 Protein is greater than 90% as determined by SEC-HPLC.