• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human VEGF121, His Tag at 2 ug/ml (100 ul/Well). Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 48.8 ng/ml determined by ELISA.
  • Amino Acid Range: Ala27-Arg147
Catalog #VEG-HM421
Price
Regular price $379.50
Regular price Sale price $379.50
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human VEGF121 Protein (VEG-HM421) Summary

Product Description

 Recombinant Human VEGF121 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg147.

Product Background

 Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Non-biotinylated Human VEGF121 Protein (VEG-HM421) Specifications/Details

Protein
 VEGF121
Synonyms
 VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Arg147
Molecular Weight
 The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF121, His Tag at 2 ug/ml (100 ul/Well). Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 48.8 ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 250 mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human VEGF121, His Tag at 2 ug/ml (100 ul/Well). Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 48.8 ng/ml determined by ELISA. Immobilized Human VEGF121, His Tag at 2 ug/ml (100 ul/Well). Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 48.8 ng/ml determined by ELISA.
Immobilized Human VEGF121, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF121 Antibody, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA. Immobilized Human VEGF121, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF121 Antibody, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA.
Human VEGF121 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%. Human VEGF121 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...