• Express System: HEK293
  • Product Tag: C-Llama IgG2b Fc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 59.5ng/ml determined by ELISA.
  • Amino Acid Range: Ala27-Arg191
Catalog #VEG-HM665-UL
Price
Regular price $36300
Regular price Sale price $36300
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Human VEGF165 Protein,-Ultra Low Endotoxin (VEG-HM665-UL) Summary

Product Description

 Recombinant Human VEGF165 Protein is expressed from HEK293 with Llama IgG2b Fc tag at the C-terminus.It contains Ala27-Arg191.

Product Background

 Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Human VEGF165 Protein,-Ultra Low Endotoxin (VEG-HM665-UL) Specifications/Details

Protein
 VEGF165
Synonyms
 VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165;VEGF165;Vascular endothelial growth factor-165; Vascular endothelial growth factor 165
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Arg191
Molecular Weight
 The protein has a predicted MW of 46.47 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 59.5ng/ml determined by ELISA.
Product Tag
 C-Llama IgG2b Fc
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 59.5ng/ml determined by ELISA. Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 59.5ng/ml determined by ELISA.
Immobilized Human VEGF165, Llama IgG2b Fc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, His Tag with the EC50 of 0.33ug/ml determined by ELISA. Immobilized Human VEGF165, Llama IgG2b Fc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, His Tag with the EC50 of 0.33ug/ml determined by ELISA.
Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC. The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...