• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag (Cat. VGF-HM2R2) with the EC50 of 2.7ng/ml determined by ELISA.
  • Amino Acid Range: Ala27-Arg191
Catalog #VEG-HM165
Price
Regular price $1,000.00
Regular price Sale price $1,000.00
/
Size
Avantor VWR Fisher Scientific

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Human VEGF165 Protein (VEG-HM165) Summary

Product Description

Recombinant Human VEGF165 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala27-Arg191.

Product Background

Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human VEGF165 Protein (VEG-HM165) Specifications/Details

Protein
VEGF165
Synonyms
VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala27-Arg191
Molecular Weight
The protein has a predicted MW of 20.77 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag (Cat. VGF-HM2R2) with the EC50 of 2.7ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag (Cat. VGF-HM2R2) with the EC50 of 2.7ng/ml determined by ELISA. Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag (Cat. VGF-HM2R2) with the EC50 of 2.7ng/ml determined by ELISA.
Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
Immobilized Human VEGF165, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 1.0ng/ml determined by ELISA. Immobilized Human VEGF165, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 1.0ng/ml determined by ELISA.
Immobilized Human VEGF165, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGFA Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. Immobilized Human VEGF165, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGFA Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC. The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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