Product Description |
Recombinant Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191. |
Product Background |
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. |
Product Category |
Recombinant Protein |
Target |
VEGF165 |
Synonyms |
VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala27-Arg191 |
Molecular Weight |
The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human VEGF165 at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human VEGF165 at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA (QC Test). |
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Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. |
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Immobilized Human VEGF165, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA. |
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Human VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. |
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