Human VEGF165 Protein (VEG-HM465)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human VEGF165 at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala27-Arg191

Catalog: VEG-HM465

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Product Description Recombinant Human VEGF165 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala27-Arg191.
Product Category Recombinant Protein
Target VEGF165
Synonyms VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
Gene/Protein ID P15692-4
Species Human
Exact Sequence Ala27-Arg191
Molecular Weight The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-32 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human VEGF165 at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF165 at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
Human VEGF165 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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