Name: Human VEGF165 Protein (VEG-HM665)
Brand: KactusBio
SKU: VEG-HM665-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-Llama IgG2b Fc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 69.9ng/ml determined by ELISA.
Amino Acid Range: Ala27-Arg191

Catalog #VEG-HM665

Size

100UG

500UG

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Human VEGF165 Protein (VEG-HM665) Summary

Product Description	Recombinant Human VEGF165 Protein is expressed from HEK293 with Llama IgG2b Fc tag at the C-terminus.It contains Ala27-Arg191.
Product Background	Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human VEGF165 Protein (VEG-HM665) Specifications/Details

Protein	VEGF165
Synonyms	VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165
Accession	P15692-4
Species	Human
Biotinylated	no
Amino Acid Range	Ala27-Arg191
Molecular Weight	The protein has a predicted MW of 46.47 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 69.9ng/ml determined by ELISA.
Product Tag	C-Llama IgG2b Fc
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 69.9ng/ml determined by ELISA.
	Immobilized Human VEGF165, Llama IgG2b Fc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, His Tag with the EC50 of 0.33ug/ml determined by ELISA.
	Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.

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