• Express System: HEK293
  • Product Tag: C-Llama IgG2b Fc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 59.5ng/ml determined by ELISA.
  • Amino Acid Range: Ala27-Arg191
Catalog #VEG-HM665
Price
Regular price $330.00
Regular price Sale price $330.00
/
Size
Avantor VWR Fisher Scientific

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Human VEGF165 Protein (VEG-HM665) Summary

Product Description

 Recombinant Human VEGF165 Protein is expressed from HEK293 with Llama IgG2b Fc tag at the C-terminus.It contains Ala27-Arg191.

Product Background

 Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Human VEGF165 Protein (VEG-HM665) Specifications/Details

Protein
 VEGF165
Synonyms
 VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165;VEGF165;Vascular endothelial growth factor-165; Vascular endothelial growth factor 165
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Arg191
Molecular Weight
 The protein has a predicted MW of 46.47 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 59.5ng/ml determined by ELISA.
Product Tag
 C-Llama IgG2b Fc
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 59.5ng/ml determined by ELISA. Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 59.5ng/ml determined by ELISA.
Immobilized Human VEGF165, Llama IgG2b Fc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, His Tag with the EC50 of 0.33ug/ml determined by ELISA. Immobilized Human VEGF165, Llama IgG2b Fc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, His Tag with the EC50 of 0.33ug/ml determined by ELISA.
Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC. The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...