Human Vγ9Vδ2 TCR Protein (TCR-HM1GV)

Immobilized Human Vγ9Vδ2 TCR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-γ9δ2 TCR Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.

Human Vγ9Vδ2 TCR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Vγ9Vδ2 TCR is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human Vγ9Vδ2 TCR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-γ9δ2 TCR Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
Amino Acid Range: Ala21-Val121(TRGV9) & Ala20-Thr115(TRDV2)

Catalog #TCR-HM1GV

Price

$420⁰⁰

Size

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Human Vγ9Vδ2 TCR Protein (TCR-HM1GV) Summary

Product Description

Recombinant Human Vγ9Vδ2 TCR Protein is expressed from HEK293, with a His tag at the C-terminus of the TRGV9 chain.It contains Ala21-Val121(TRGV9) & Ala20-Thr115(TRDV2).

Product Background

Human Vγ9Vδ2 T cells, a major innate-like peripheral T cell subset, can behave as cellular adjuvants to rally a wide range of immune reactions against cancer cells mediated by innate and adaptive immune effector cells, including B cells, neutrophils, and NK cells. Vγ9Vδ2 T cells can provide B-cell help to promote antibody production and immunoglobulin class switching. Vγ9Vδ2 T cells are very attractive candidates for cell-based immunotherapy in cancers.

γ9δ2 Specifications

Protein	γ9δ2
Synonyms	Vγ9Vδ2 TCR
Accession	Q99603(TRGV9)& A0JD36(TRDV2)
Species	Human
Biotinylated	no
Amino Acid Range	Ala21-Val121(TRGV9) & Ala20-Thr115(TRDV2)
Molecular Weight	The protein has a predicted MW of 21.32 kDa (TRGV9) and 20.71 kDa (TRDV2). Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Vγ9Vδ2 TCR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-γ9δ2 TCR Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Vγ9Vδ2 TCR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-γ9δ2 TCR Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
	Human Vγ9Vδ2 TCR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Vγ9Vδ2 TCR is greater than 90% as determined by SEC-HPLC.