Human VLDLR Protein (VDR-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.72 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly28-Ser797

Catalog: VDR-HM101

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Product Description	Recombinant Human VLDLR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly28-Ser797.
Product Background	VLDLR cerebellar hypoplasia (VLDLR-CH) is characterized by non-progressive congenital ataxia that is predominantly truncal and results in delayed ambulation, moderate-to-profound intellectual disability, dysarthria, strabismus, and seizures.VLDLR-CH is inherited in an autosomal recessive manner. Carrier testing for at-risk relatives, prenatal testing for a pregnancy at increased risk and preimplantation genetic testing are possible when the pathogenic variants in a family are known.
Product Category	Recombinant Protein

Target	VLDLR
Synonyms	VLDL-R; VLDLR; RP11-320E16.1; CHRMQ1; FLJ35024; VLDLRCH
Accession	P98155-1
Species	Human
Amino Acid Range	Gly28-Ser797
Molecular Weight	The protein has a predicted MW of 85.9 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.72 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human VLDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.72 nM as determined in SPR assay (Biacore T200).
	The purity of Human VLDLR is greater than 95% as determined by SEC-HPLC.
	Human VLDLR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.