Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM431)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.33nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide

Catalog: MHC-HM431

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Product Description Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.

Product Background

The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1.

Product Category

Recombinant Protein

Target

HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer
Synonyms MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
Accession
MHC Form Monomer
Allele HLA-A*02:01

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide
Molecular Weight The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.33nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with an affinity constant of 0.33nM as determined in SPR assay (Biacore T200).
Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA (QC Test).
The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer was greater than 95% as determined by SEC-HPLC.
Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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