Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein (MHC-HM431T)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide
Catalog #MHC-HM431T
Regular price $750.00
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Product Description
Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide.
Product Background
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1.
Product Category
Recombinant Protein
Target
HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer
Synonyms
MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
MHC Form
Tetramer
Allele
HLA-A*02:01
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide
Molecular Weight
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity
Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody captured on Protein A chip, can bind Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag with an affinity constant of 0.18nM as determined in a SPR assay (Biacore T200).
Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test).
Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer was greater than 95% as determined by SEC-HPLC.

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