Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein (MHC-HM431T)
Catalog: MHC-HM431T
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| Product Description | Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide. |
Product Background |
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer |
| Synonyms | MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33 |
| MHC Form | Tetramer |
| Allele | HLA-A*02:01 |
Gene/Protein ID |
A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL |
Species |
Human |
Exact Sequence |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide |
| Molecular Weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
Activity |
Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody captured on Protein A chip, can bind Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag with an affinity constant of 0.18nM as determined in a SPR assay (Biacore T200). |
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Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test). |
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The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer was greater than 95% as determined by SEC-HPLC. |
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Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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