Human WT-1 (HLA-A*02:01) Tetramer Protein (MHC-HM431T)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human WT-1 (HLA-A*02:01) Tetramer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide

Catalog: MHC-HM431T

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Product Description Recombinant Human WT-1 (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.
Product Category Recombinant Protein
Target WT-1 (HLA-A*02:01) Tetramer
Synonyms MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
Gene/Protein ID A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL
Species Human
Exact Sequence Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity Immobilized Human WT-1 (HLA-A*02:01) Tetramer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-WT-1 (HLA-A*02:01) Antibody captured on Protein A chip, can bind Human WT-1 (HLA-A*02:01) Tetramer, His Tag with an affinity constant of 0.18nM as determined in a SPR assay (Biacore T200).
Immobilized Human WT-1 (HLA-A*02:01) Tetramer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test).
The purity of Human WT-1 (HLA-A*02:01) Tetramer was greater than 95% as determined by SEC-HPLC.
Human WT-1 (HLA-A*02:01) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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