Human YKL-40/CHI3L1 Protein (YKL-HM140)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human YKL-40, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr22-Thr383

Catalog: YKL-HM140

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Product Description Recombinant Human YKL-40/CHI3L1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Thr383.
Product Category Recombinant Protein
Target YKL-40/CHI3L1
Synonyms BRP39; CGP-39; GP-39; Chil1; ASRT7; FLJ38139; HC-gp39; YKL-40; YKL40; YYL-40; AW208766; CHI3L1; Chitinase 3 like 1
Gene/Protein ID P36222
Species Human
Exact Sequence Tyr22-Thr383
Molecular Weight The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human YKL-40, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human YKL-40, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA.
The purity of Human YKL-40 is greater than 95% as determined by SEC-HPLC.
Human YKL-40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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