Product Description |
Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Arg104-Glu309. |
Product Background |
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner. |
Product Category |
Recombinant Protein |
Protein |
4-1BB Ligand/TNFSF9 |
Synonyms |
41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Arg104-Glu309 |
Molecular Weight |
The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 0.1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). |
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|
Mouse 4-1BB Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse 4-1BB Ligand is greater than 95% as determined by SEC-HPLC. |
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