• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Arg104-Glu309
Catalog #BBL-MM241
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Mouse 4-1BB Ligand/TNFSF9 Protein (BBL-MM241) Summary

Product Description

 Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Arg104-Glu309.

Product Background

 The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.

Product Category

 Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Mouse 4-1BB Ligand/TNFSF9 Protein (BBL-MM241) Specifications/Details

Protein
 4-1BB Ligand/TNFSF9
Synonyms
 41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Arg104-Glu309
Molecular Weight
 The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test).
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test).
Mouse 4-1BB Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse 4-1BB Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse 4-1BB Ligand is greater than 95% as determined by SEC-HPLC. The purity of Mouse 4-1BB Ligand is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...