Mouse 4-1BB/TNFRSF9 Protein (BB4-MM141)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 0.51ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val24-Leu211

Catalog: BB4-MM141

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Product Description Recombinant Mouse 4-1BB/TNFRSF9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Leu211.

Product Background

4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.

Product Category

Recombinant Protein

Target

4-1BB/TNFRSF9
Synonyms CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA

Gene/Protein ID

NP_001070976.1

Species

Mouse

Exact Sequence

Val24-Leu211
Molecular Weight The protein has a predicted MW of 21.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 0.51ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 0.51ug/ml determined by ELISA.
The purity of Mouse 4-1BB is greater than 95% as determined by SEC-HPLC.
Mouse 4-1BB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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