Mouse 4-1BB/TNFRSF9 Protein (BB4-MM141)
Mouse 4-1BB/TNFRSF9 Protein (BB4-MM141)
Mouse 4-1BB/TNFRSF9 Protein (BB4-MM141)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
  • Amino Acid Range: Val24-Leu211
Catalog #BB4-MM141
Regular price $297.00
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Mouse 4-1BB/TNFRSF9 Protein (BB4-MM141) Summary

Product Description

Recombinant Mouse 4-1BB/TNFRSF9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Leu211.

Product Background

4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Mouse 4-1BB/TNFRSF9 Protein (BB4-MM141) Specifications/Details

Protein
4-1BB/TNFRSF9
Synonyms
CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Val24-Leu211
Molecular Weight
The protein has a predicted MW of 21.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. Immobilized Mouse 4-1BB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Mouse 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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