Mouse Adiponectin/Acrp30 Protein (ADI-MM101) Summary
Product Description |
Recombinant Mouse Adiponectin/Acrp30 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu18-Asn247. |
Product Background |
Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation.Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse Adiponectin/Acrp30 Protein (ADI-MM101) Specifications/Details
Protein |
Adiponectin/Acrp30 |
Synonyms |
ACDC; Acrp30; ADPN; Adiponectin; AdipoQ; ADIPQTL1; APM1APM-1; GBP28; GBP28apM1; APM-1; APM1 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Glu18-Asn247 |
Molecular Weight |
The protein has a predicted MW of 25.9 kDa. Due to glycosylation, the protein migrates to 32-35 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse Adiponectin/Acrp30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse Adiponectin/Acrp30 is greater than 95% as determined by SEC-HPLC. |
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