Mouse ANGPTL3/Angiopoietin-like 3 Protein (ANG-MM103)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse ANGPTL3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser17-Thr455
Catalog #ANG-MM103
Regular price $297.00
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Product Description

Recombinant Mouse ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser17-Thr455.

Product Background

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

ANGPTL3/Angiopoietin-like 3

Synonyms

ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Ser17-Thr455

Molecular Weight

The protein has a predicted MW of 51.81 kDa. Due to furin cleavage site, the protein migrates to 30-35 kDa, 43-48 kDa and 60-75 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse ANGPTL3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse ANGPTL3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. Immobilized Mouse ANGPTL3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
Mouse ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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