Mouse ANGPTL3/Angiopoietin-like 3 Protein (ANG-MM103)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse ANGPTL3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser17-Thr455
Catalog #ANG-MM103
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Product Description
 Recombinant Mouse ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser17-Thr455.
Product Background
 ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.
Product Category
 Recombinant Protein
Target
 ANGPTL3/Angiopoietin-like 3
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Ser17-Thr455
Molecular Weight
 The protein has a predicted MW of 51.81 kDa. Due to furin cleavage site, the protein migrates to 30-35 kDa, 43-48 kDa and 60-75 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse ANGPTL3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse ANGPTL3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
Mouse ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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