Mouse APCDD1 Protein (APD-MM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Leu27-Thr492

Catalog: APD-MM101

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Product Description Recombinant Mouse APCDD1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu27-Thr492.

Product Background

 Adenomatosis polyposis coli downregulated 1 (APCDD1), a negative regulator of Wnt signaling, was examined to understand detailed mechanisms underlying Wnt signaling tooth development. In situ hybridization showed that Apcdd1 was expressed in the condensed mesenchyme at the bud stage, and in the inner enamel epithelium (IEE), including enamel knot (EK) at the cap stage. APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE.

Product Category

 Recombinant Protein

Target

 APCDD1
Synonyms Apcdd1; Drapc1; B7323; FP7019; HHS; HTS; HYPT1

Gene/Protein ID

 Q3U128

Species

 Mouse

Exact Sequence

 Leu27-Thr492
Molecular Weight The protein has a predicted MW of 54.4 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Mouse APCDD1 is greater than 95% as determined by SEC-HPLC.
Mouse APCDD1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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