Mouse APRIL/TNFSF13 Protein (APR-MM110)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala96-Leu241

Catalog: APR-MM110

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Product Description Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala96-Leu241.

Product Background

The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.

Product Category

Recombinant Protein

Target

APRIL/TNFSF13
Accession

Species

Mouse

Exact Sequence

Ala96-Leu241
Molecular Weight The protein has a predicted MW of 43.20 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA.
The purity of Mouse APRIL is greater than 95% as determined by SEC-HPLC.
Mouse APRIL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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