Mouse APRIL/TNFSF13 Protein (APR-MM110)
Mouse APRIL/TNFSF13 Protein (APR-MM110)
Mouse APRIL/TNFSF13 Protein (APR-MM110)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala96-Leu241
Catalog #APR-MM110
Regular price $315.00
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Product Description
Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala96-Leu241.
Product Background
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Product Category
Recombinant Protein
Target
APRIL/TNFSF13
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ala96-Leu241
Molecular Weight
The protein has a predicted MW of 43.20 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA.
Mouse APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse APRIL is greater than 95% as determined by SEC-HPLC.

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