Mouse APRIL/TNFSF13 Protein (APR-MM110) Summary
Product Description |
Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala96-Leu241. |
Product Background |
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse APRIL/TNFSF13 Protein (APR-MM110) Specifications/Details
Protein |
APRIL/TNFSF13 |
Synonyms |
CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala96-Leu241 |
Molecular Weight |
The protein has a predicted MW of 43.20 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA. |
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Mouse APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind Mouse APRIL, hFc Tag with an affinity constant of 7.09 nM as determined in BLI assay (Gator® Prime). |
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The purity of Mouse APRIL is greater than 95% as determined by SEC-HPLC. |
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