Mouse APRIL/TNFSF13 Protein (APR-MM113)
Mouse APRIL/TNFSF13 Protein (APR-MM113)
Mouse APRIL/TNFSF13 Protein (APR-MM113)
  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala96-Leu241
Catalog #APR-MM113
Regular price $315.00
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Product Description

Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ala96-Leu241.

Product Background

The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.

Product Category

Recombinant Protein / Antibody discovery

Protein

APRIL/TNFSF13

Synonyms

CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Ala96-Leu241

Molecular Weight

The protein has a predicted MW of 18.53 kDa. Due to glycosylation, the protein migrates to 24-30 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Flag

Expression System

HEK293

Purity

> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
Mouse APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Mouse APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Mouse APRIL is greater than 90% as determined by SEC-HPLC. The purity of Mouse APRIL is greater than 90% as determined by SEC-HPLC.

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