Mouse APRIL/TNFSF13 Protein (APR-MM113)
Mouse APRIL/TNFSF13 Protein (APR-MM113)
Mouse APRIL/TNFSF13 Protein (APR-MM113)
Mouse APRIL/TNFSF13 Protein (APR-MM113)
Mouse APRIL/TNFSF13 Protein (APR-MM113)
Mouse APRIL/TNFSF13 Protein (APR-MM113)
  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala96-Leu241
Catalog #APR-MM113
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Product Description
 Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ala96-Leu241.
Product Background
 The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Product Category
 Recombinant Protein
Target
 APRIL/TNFSF13
Accession
Species
 Mouse
Amino Acid Range
 Ala96-Leu241
Molecular Weight
 The protein has a predicted MW of 18.53 kDa. Due to glycosylation, the protein migrates to 24-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His-Flag
Express System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
Mouse APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Mouse APRIL is greater than 90% as determined by SEC-HPLC.
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