Name: Mouse ARTN Protein (ARN-MM201)
Brand: KactusBio
SKU: ARN-MM201-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 54.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala112-Gly224

Catalog #ARN-MM201

Size

100UG

500UG

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Product Description	Recombinant Mouse ARTN Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala112-Gly224.
Product Background	Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	ARTN
Synonyms	Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN
Accession	Q9Z0L2-1
Species	Mouse
Biotinylated	no
Amino Acid Range	Ala112-Gly224
Molecular Weight	The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 54.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM Glycine,150mM NaCl (pH 3.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 50mM Glycine,150mM NaCl (pH 3.2). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 54.7ng/ml determined by ELISA (QC Test).
	Mouse ARTN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.