Mouse B7-2/CD86 Protein (B72-MM186)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Val24-Lys244

Catalog: B72-MM186

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Product Description	Recombinant Mouse B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val24-Lys244.
Product Background	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Product Category	Recombinant Protein

Target	B7-2/CD86
Accession	P42082-1
Species	Mouse
Amino Acid Range	Val24-Lys244
Molecular Weight	The protein has a predicted MW of 26.19 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
	The purity of Mouse B7-2 is greater than 95% as determined by SEC-HPLC.
	Mouse B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.