Mouse B7-2/CD86 Protein (B72-MM186)
Mouse B7-2/CD86 Protein (B72-MM186)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val24-Lys244
Catalog #B72-MM186
Regular price $135.00
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Product Description

Recombinant Mouse B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val24-Lys244.

Product Background

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

Product Category

Recombinant Protein

Protein

B7-2/CD86

Synonyms

CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Val24-Lys244

Molecular Weight

The protein has a predicted MW of 26.19 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
Mouse B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse B7-2 is greater than 95% as determined by SEC-HPLC. The purity of Mouse B7-2 is greater than 95% as determined by SEC-HPLC.

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