|Recombinant Mouse B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val24-Lys244.
|B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
|The protein has a predicted MW of 26.19 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Tris-Bis PAGE result.
|Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped with dry ice.
|Supplied as 0.22 um filtered solution in PBS (pH 7.4).
|Stability And Storage
|Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.