Mouse B7-2/CD86 Protein (B72-MM186)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val24-Lys244

Catalog: B72-MM186

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Product Description Recombinant Mouse B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val24-Lys244.

Product Background

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

Product Category

Recombinant Protein

Target

B7-2/CD86

Gene/Protein ID

P42082-1

Species

Mouse

Exact Sequence

Val24-Lys244
Molecular Weight The protein has a predicted MW of 26.19 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
The purity of Mouse B7-2 is greater than 95% as determined by SEC-HPLC.
Mouse B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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