Mouse BAFF/TNFSF13B/CD257 Protein (BAF-MM301)
  • Express System: HEK293
  • Product Tag: N-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse BAFF, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
  • Amino Acid Range: Ala127-Leu309
Catalog #BAF-MM301
Regular price $363.00
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Mouse BAFF/TNFSF13B/CD257 Protein (BAF-MM301) Summary

Product Description

Recombinant Mouse BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-terminus.It contains Ala127-Leu309.

Product Background

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse BAFF/TNFSF13B/CD257 Protein (BAF-MM301) Specifications/Details

Protein
BAFF/TNFSF13B/CD257
Synonyms
BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ala127-Leu309
Molecular Weight
The protein has a predicted MW of 47.22 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse BAFF, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
Product Tag
N-mFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse BAFF, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA. Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse BAFF, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
Immobilized Mouse BAFF, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA. Immobilized Mouse BAFF, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA.
Immobilized Mouse BAFF, mFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. Immobilized Mouse BAFF, mFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
Mouse BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 39.20 nM as determined in BLI assay (Gator® Prime). Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 39.20 nM as determined in BLI assay (Gator® Prime).
Loaded Mouse BAFFR, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 2.56 nM as determined in BLI assay (Gator® Prime). Loaded Mouse BAFFR, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 2.56 nM as determined in BLI assay (Gator® Prime).
The purity of Mouse BAFF is greater than 90% as determined by SEC-HPLC. The purity of Mouse BAFF is greater than 90% as determined by SEC-HPLC.
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