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Mouse BAFF/TNFSF13B/CD257 Protein (BAF-MM301) Summary
Product Description |
Recombinant Mouse BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-terminus.It contains Ala127-Leu309. |
Product Background |
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Mouse BAFF/TNFSF13B/CD257 Protein (BAF-MM301) Specifications/Details
Protein |
BAFF/TNFSF13B/CD257 |
Synonyms |
BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala127-Leu309 |
Molecular Weight |
The protein has a predicted MW of 47.22 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse BAFF, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA. |
Product Tag |
N-mFc |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Mouse BAFFR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse BAFF, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA. |
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Immobilized Mouse BAFF, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA. |
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Immobilized Mouse BAFF, mFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. |
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Mouse BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 39.20 nM as determined in BLI assay . |
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Loaded Mouse BAFFR, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 2.56 nM as determined in BLI assay . |
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The purity of Mouse BAFF is greater than 85% as determined by SEC-HPLC. |
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